Department of Chemistry

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Stephanie Rankin-Turner

Assistant Professor

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Eberly Hall
Pittsburgh, PA 15260

Research Overview

The human body emits a complex blend of hundreds of volatile organic compounds (VOCs), collectively known as the volatilome, through exhaled breath, skin, and bodily fluids. Influenced by factors such as disease, nutrition, environment, and the microbiome, the chemical study of the volatilome can provide unparalleled insight into human health and physiology.

 

The Rankin-Turner lab uses ambient ionization mass spectrometry and gas chromatography/mass spectrometry to study the human volatilome. Employing comprehensive profiling of whole body, skin, and exhaled breath volatiles, our research aims to elucidate how the chemistry of the human body changes in response to factors such as disease, infection and the environment. We can then leverage this information to develop new tools for the study and non-invasive diagnosis of diverse health conditions, bridging the gap between laboratory research and clinical practice. Our research incorporates aspects of analytical chemistry, clinical chemistry, biochemistry, and data science. Members of the Rankin-Turner lab gain expertise in mass spectrometry, separation science, use and modification of analytical instrumentation, analysis of complex datasets, and managing human subjects research.

 

 

Publications

“Ambient ionisation mass spectrometry for drug and toxin analysis: A review of the recent literature.,” A. Henderson, L. M. Heaney, S. Rankin-Turner Drug Testing & Analysis 2024
“Analysis of human skin sebum and animal meats by heat pulse desorption/mass spectrometry using proximity corona discharge ionization,” K. Hiraoka, H. Shimada, K. Kinoshita, S. Rankin-Turner, S. Ninomiya Analytical Biochem 2023, 115249
“Human scent guides mosquito thermotaxis and host selection under naturalistic conditions,” D. Giraldo, S. Rankin-Turner, A. Corver, G. M. Tauxe, A. L. Gao, D. M Jackson, L. Simubali, C. Book, J. C. Stevenson, P. E. Thuma, A. Gordus, R. C. McCoy, M. M. Mburu, E. Simulundu, C. J. McMeniman Current Biology 2023, 2367-2382
“Applications of ambient ionization mass spectrometry in 2022: An annual review,” S. Rankin-Turner, P. Sears, L. M. Heaney Analytical Science Advances 2023, 133-155
“Electrospray droplet impact/secondary ion mass spectrometry (EDI/SIMS) applied to the analysis of explosives,” K. Hiraoka, R. Takaishi, S. Ninomiya, S. Rankin-Turner Int Journal of Mass Spec 2023, 116993
“Site-specific deprotonation/sodiation of gramicidin S studied by electrospray ionization/mass spectrometry,” K. Hiraoka, S. Akashi, S. Rankin-Turner, S. Ninomiya Int Journal of Mass Spec 2023, 117135
“Site-specific sodiation of peptides studied by pulsed nanoelectrospray ionization,” K. Hiraoka, D.T. Usmanov, S. Ninomiya, S. Rankin-Turner, S. Akashi Int Journal of Mass Spec 2023, 117073
“Cuticular profiling of insecticide resistant Aedes aegypti,” E. Jacobs, C. Chrissian, S. Rankin-Turner, M. Wear, E. Camacho, N. A. Broderick, C. J. McMeniman, R. E. Stark, A. Casadevall Scientific Reports 2023, 10154
“A headspace collection chamber for whole body volatilomics,” S. Rankin-Turner, C. J. McMeniman Analyst 2022, 5210-5222
“Heat Pulse Desorption of Low-Volatility Compounds by a Heated N2 Gas Pulse with Mass Spectrometry,” S. Ninomiya, H. Shimada, K. Kinoshita, S. Rankin-Turner, K. Hiraoka J. Am. Soc. Mass Spectrom 2022, 2046–2054
“Applications of ambient ionization mass spectrometry in 2021: An annual review,” S. Rankin-Turner, J. C. Reynolds, M. A. Turner, L. M. Heaney Anal. Sci. Adv 2022, 67–89
“Comparative study of H3O+ (aq) and NH4+ (aq) on electrophoresis, protonating ability, and sodiation of proteins,” K. Hiraoka, S. Ninomiya, S. Rankin-Turner, S. Akashi Int Journal of Mass Spec 2022, 116728
“Corona Discharge and Field Electron Emission in Ambient Air Using a Sharp Metal Needle: Formation and Reactivity of CO3-• and O2-•,” K. Hiraoka, S. Rankin-Turner, S. Ninomiya, H. Shimada, K. Kinoshita, S. Yamabe Mass Spectrometry 2021, A0100
“A call for the standardised reporting of factors affecting the exogenous loading of extracellular vesicles with therapeutic cargos,” S. Rankin-Turner, P. Vader, L. O'Driscoll, B. Giebel, L. M.Heaney, O. G. Davies Advanced Drug Delivery Reviews 2021, 479-491
“Applications of ambient ionization mass spectrometry in 2020: An annual review,” S. Rankin-Turner, L. M. Heaney Anal. Sci. Adv 2021, 193-212
“Using mass spectrometry to transform the assessment of sexual assault evidence,” S. Rankin-Turner, P. F. Kelly, R. S. P. King, J. C. Reynolds Forensic Chem 2020, 100262
“Point analysis of foods by sheath-flow probe electrospray ionization/mass spectrometry (sfPESI/MS) coupled with a touch sensor,” K. Hiraoka, S. Rankin-Turner, S. Ninomiya, R. Sekine, H. Wada, M, Matsumura, S. Sanada-Morimura, F. Tanaka, H. Nonami, O. Ariyada J. Agric. Food Chem 2020, 418-425
“Sheath-flow probe electrospray ionization (sfPESI) mass spectrometry for the rapid forensic analysis of human body fluids,” S. Rankin-Turner, S. Ninomiya, J. C. Reynolds, K. Hiraoka Analytical Methods 2019, 3633-3640
“Component Profiling in Agricultural Applications Using an Adjustable Acupuncture Needle for Sheath-Flow Probe Electrospray Ionization/Mass Spectrometry,” K. Hiraoka, S. Rankin-Turner, S. Ninomiya, H. Wada, H. Nakano, M. Matsumura, S. Sanada-Morimura, F. Tanaka, H. Nonami J. Agric. Food Chem 2019, 3275–3283
“Transforming Presumptive Forensic Testing: In-situ Identification and Age Estimation of Human Bodily Fluids,” S. Rankin-Turner, M. A. Turner, P. F. Kelly, R. S. P. King, J. C. Reynolds Chemical Science 2019, 1064-1069